Recombinant adeno-associated virus vector related impurities.
- Author:
Yong DIAO
1
;
Qizhao WANG
;
Weidong XIAO
;
Rui'an XU
Author Information
1. Engineering Research Center of Molecular Medicine, Ministry of Education, Institute of Molecular Medicine, Huaqiao University, Quanzhou 362021, China. diaoyong@hqu.edu.cn
- Publication Type:Journal Article
- MeSH:
Capsid Proteins;
isolation & purification;
Dependovirus;
genetics;
isolation & purification;
physiology;
Genetic Therapy;
methods;
Genetic Vectors;
genetics;
isolation & purification;
Recombination, Genetic;
Virion;
isolation & purification;
Virus Assembly;
genetics;
Virus Replication;
genetics
- From:
Chinese Journal of Biotechnology
2011;27(5):717-723
- CountryChina
- Language:Chinese
-
Abstract:
Recombinant adeno-associated virus (rAAV)-based vectors that can stably express therapeutic genes in vivo without detectable side-effect have shown great promise for human gene therapy. A major challenge for translation of promising research to clinical development is how to establish clinically compatible purification methods in separating rAAV from potentially pathogenic impurities, especially rAAV vector-related impurities, a class of impurities corresponding to AAV particles that closely resemble bona fide vectors and are difficult to remove. In this review we summarize the assembly process of rAAV vector-related impurities and their characteristics differed with rAAV vectors, and evaluate several current technologies to prevent their formation or separate them from rAAV stocks.
