Immunological efficiency induced by HIV-1 p24 DNA combined with P24 protein.
- Author:
Qingtao WANG
1
;
Yuhai CHEN
;
Shijuan GAO
;
Wei JIANG
;
Liping SONG
;
Wenlin HUANG
Author Information
1. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
- Publication Type:Journal Article
- MeSH:
AIDS Vaccines;
genetics;
immunology;
Animals;
DNA;
genetics;
immunology;
HEK293 Cells;
HIV Core Protein p24;
genetics;
immunology;
Humans;
Immunization;
Mice;
Mice, Inbred BALB C;
Vaccines, DNA;
genetics;
immunology
- From:
Chinese Journal of Biotechnology
2011;27(5):773-780
- CountryChina
- Language:Chinese
-
Abstract:
New strategies to improve vaccine efficacy against human immunodeficiency virus type 1 (HIV-1) are still required. DNA vaccines, exhibiting potential advantages over conventional vaccines for their simplicity and versatility, can induce specific humoral and cellular immune responses. We developed a recombinant pVAX1 DNA vector carrying p24 gene of HIV-1. The results showed that pVAX1 mediated gene possessed the ability of effective expression in both transfected 293T cells and BALB/c mice. And pVAX1-p24 DNA prime and boost immunization can induce significant P24-specific humoral immune responses and cellular immune responses in BALB/c mice. Furthermore, immunization with pVAX1-p24 DNA prime and protein boost induced 7.3 to 8.0-fold greater p24-specific humoral responses than pVAX1-p24 DNA prime and boost, while the cellular immune responses induced by combined immunization was lower. The results suggested that pVAX1-p24 DNA and P24 protein vaccine is a promising HIV-1 vaccine, and the selections of the immunization strategies are important for the immunization results.
