Development of a killed but metabolically active anthracis vaccine candidate strain.
- Author:
Fei SHEN
1
;
Shengling YUAN
;
Dewen ZHAN
;
Yanchun WANG
;
Min REN
;
Haoxia TAO
;
Peng WANG
;
Lingchun WANG
;
Dongsheng CHEN
;
Chunjie LIU
Author Information
1. College of Life Sciences, Anhui Normal University, Wuhu 241000, China.
- Publication Type:Journal Article
- MeSH:
Anthrax;
immunology;
microbiology;
prevention & control;
Anthrax Vaccines;
genetics;
immunology;
radiation effects;
Bacillus anthracis;
genetics;
immunology;
Gene Knockout Techniques;
Methoxsalen;
pharmacology;
Ultraviolet Rays;
Vaccines, Inactivated;
genetics;
immunology
- From:
Chinese Journal of Biotechnology
2011;27(5):781-789
- CountryChina
- Language:Chinese
-
Abstract:
Anthrax is a zoonosis caused by Bacillus anthracis, which seriously affects human health. In recent years, a special phenomenon is found that the metabolic active of a bacterium remains after it is killed. To development of a KBMA (killed but metabolically active) Bacillus anthracis vaccine candidate strain, a plasmid pMAD and a recombinase system Cre-loxP were used to knockout the uvrAB gene of B. anthracis AP422 which lacks both of two plasmids pXO1 and pXO2. The results of PCR and RT-PCR shows that uvrAB genes were deleted from B. anthracis AP422 chromosome successfully. The constructed B. anthracis AP422deltauvrAB was inactivated by photochemical treatment (PCT) including an exposure in a long-wave-length ultraviolet (UVA) light and a treatment of 8-Methoxypsoralen (8-MOP), then the metabolic activity were detected by the method of MTS. The results showed that the killed B. anthracis AP422deltauvrAB maintained a highly metabolic activity for at least 4 hours, showing a state of KBMA. The KBMA strain of B. anthracis AP422deltauvrAB provides the prospective vaccine candidate strain for anthrax.
