Preparation and application of a colloidal gold strip to detect the rabies antibody.
- Author:
Tiecheng WANG
1
;
Tao ZHANG
;
Songtao YANG
;
Hualei WANG
;
Yuwei GAO
;
Wei SUN
;
Xiaoxia JIN
;
Pingsen ZHAO
;
Na FENG
;
Geng HUANG
;
Xiaohuan ZOU
;
Xianzhu XIA
Author Information
1. Key Laboratory of Jilin Provice for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130122, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Neutralizing;
analysis;
blood;
Antibodies, Viral;
analysis;
blood;
Dogs;
Gold Colloid;
Immunochromatography;
methods;
Rabies;
prevention & control;
veterinary;
Rabies Vaccines;
immunology;
Rabies virus;
immunology;
Reagent Strips;
Sensitivity and Specificity;
Vaccination
- From:
Chinese Journal of Biotechnology
2011;27(5):799-804
- CountryChina
- Language:Chinese
-
Abstract:
To develop a specific, rapid, and convenient immunochromatography assay (ICA) to detect the rabies antibody in clinical sample from immuned dogs by rabies vaccine. Colloidal gold particles labeled with purified rabies virus (CVS11) were used as the detector reagent. The staphylococcal protein A (SPA) and pured rabbit anti-rabies virus IgG were blotted on the test and control regions of nitrocellulose membrane. Then the strip was assembled with sample pad, absorbing pad, and dorsal shield. The assay samples (261 dog's serum) were collected from Wildlife Rabies Disease Diagnostic Laboratories of Ministry of Agriculture in China, Institute of Military Veterinary, Academy of Military Medical Sciences and other six provinces, including rabies virus positive and negative serum. The performance of the strip was compared to fluorescent antibody virus neutralization test. The neutralizing antibody titer could be detected above 0.5 IU. The strip did not change of performance when stored at room temperature for 12 months. It may offer reference of neutralizing antibody titer level after dogs immuned rabies vaccine and determin whether the dogs need to be immuned again.
