Preparation and characterization of follwing the national standard anti-Brucella abortus serum, bovine.
- Author:
Cui LI
1
;
Fushi GUAN
;
Zhihong DAI
;
Hui JIANG
;
Fang WEN
;
Lianshou LU
;
Zaishi WANG
Author Information
1. China Institute of Veterinary Drug Control, Beijing 100081, China.
- Publication Type:Journal Article
- MeSH:
Agglutination Tests;
veterinary;
Animals;
Antibodies, Bacterial;
blood;
Antigens, Bacterial;
immunology;
Brucella abortus;
immunology;
Brucellosis, Bovine;
diagnosis;
Cattle;
China;
Complement Fixation Tests;
veterinary;
Immune Sera;
Reference Standards
- From:
Chinese Journal of Biotechnology
2011;27(5):812-816
- CountryChina
- Language:Chinese
-
Abstract:
To prepare anti-Brucella abortus serum used for calibrate the agglutination test follwing the national standard, 4 anti-Brucella abortus sera were obtained from 4 cows infected with Brucella abortus naturally. By potency testing, the third serum was selected. Sterility, vaccum degree, residual moisture, uniformity and stability of this standard material were tested and proved to meet the national standard. Referring to the international standard, RBT (Rose-Bengal plate agglutination test), SAT (standard tube agglutination) and CFT (complement fixation test) titers of this standard material were measured to be 1:160 "+" 1:2 400 "++" and 1:800 "++", which are identical with the collaborative assay results. International unit of the standard material is 4 000 IU/mL.
