Applications and perspectives of DNA stable-isotope probing in metagenomics: a review.
- Author:
Wei LIU
1
;
Xiao WEI
;
Jing YUAN
;
Liuyu HUANG
Author Information
1. Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
- Publication Type:Journal Article
- MeSH:
Animals;
DNA;
genetics;
DNA Probes;
chemistry;
genetics;
metabolism;
DNA, Bacterial;
chemistry;
genetics;
metabolism;
Humans;
Isotope Labeling;
methods;
Metagenomics;
methods;
Molecular Probe Techniques;
Sequence Analysis, DNA;
methods
- From:
Chinese Journal of Biotechnology
2011;27(4):539-545
- CountryChina
- Language:Chinese
-
Abstract:
DNA stable-isotope probing (DNA-SIP) is a recently developed method with which the incorporation of stable isotope from a labeled substrate is used to identify the function of microorganisms in the environment. The technique has now been used in conjunction with metagenomics to establish links between microbial identity and particular metabolic functions. The combination of DNA-SIP and metagenomics not only permits the detection of rare low-abundance species from metagenomic libraries but also facilitates the detection of novel enzymes and bioactive compounds. We summarize recent progress in SIP-metagenomic techniques and applications and discuss prospects for this combined approach in environmental microbiology and biotechnology.
