Screening proteins interacting with infectious bursa disease virus Gt VP2 from cDNA library of B lymphoid cells of the bursa of Fabricius.
- Author:
Yulong GAO
1
;
Fenfen SUN
;
Lei HOU
;
Honglei GAO
;
Xiaole QI
;
Di LIU
;
Yuping HUA
;
Xiaomei WANG
Author Information
1. Postdoctoral Research Center, Heilongjiang Academy of Agriculture Science, Harbin 150086, China.
- Publication Type:Journal Article
- MeSH:
Animals;
B-Lymphocytes;
metabolism;
virology;
Bursa of Fabricius;
metabolism;
Chickens;
DNA, Mitochondrial;
metabolism;
Gene Library;
Infectious bursal disease virus;
Protein Binding;
Protein Interaction Mapping;
Receptors, Virus;
metabolism;
Tumor Suppressor Protein p53;
metabolism;
Two-Hybrid System Techniques;
Viral Structural Proteins;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2011;27(4):561-565
- CountryChina
- Language:Chinese
-
Abstract:
To screen the interactive proteins with IBDV Gt VP2 protein from cDNA library of B Lymphoid cells of the bursa of Fabricius. The expression cDNA library plasmids was transformed to the yeast competent cells, which have the bait plasmid-Gt VP2. After testing for growth in synthetic complete medium lacking histidine and uracil and for production of beta-galactosidase (X-gal), we obtained 16 positive clones. We searched the gene sequences of positive clones in the NCBI website. The blast results showed that five positive clones were the gallus sequences. They were Gallus gallus breed mitochondrial DNA, O_G1cNAc transferase, Tumor protein p53 binding protein, Stathmin and Chondroitin sulfate Ga1NAcT-2, respectively. This study is helpful for the further identifying the receptors of IBDV in B Lymphoid cells of the bursa of Fabricius.
