Cloning and expression of Staphylococcus aureus surface protein Isdb and its immune experiment in mice.
- Author:
Jinzhu MA
1
;
Yudong CUI
;
Jing ZHANG
;
Zhanbo ZHU
;
Fanze PIAO
Author Information
1. College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Bacterial;
blood;
Cation Transport Proteins;
biosynthesis;
genetics;
immunology;
Cloning, Molecular;
Escherichia coli;
genetics;
metabolism;
Female;
Genetic Vectors;
genetics;
Immunization;
Male;
Mice;
Recombinant Proteins;
biosynthesis;
genetics;
immunology;
Staphylococcal Infections;
immunology;
prevention & control
- From:
Chinese Journal of Biotechnology
2011;27(4):566-571
- CountryChina
- Language:Chinese
-
Abstract:
In order to characterize the immunogenicity and immunoprotection of the Staphylococcus aureus (S. aureus) surface Isdb, we amplified Isdb gene from S. aureus Wood46 strain. The isdb gene was subsequently inserted into pET32a(+) vector and the recombinant plasmid was transformed into E. coli strain BL21. The recombinant Isdb was expressed and purified. Then, we immunized mice with the purified recombinant protein. The antibody level was measured by enzyme-linked immunosorbent assay. Finally, immunized mice were challenged with S. aureus strains Wood46 and HLJ23-1. These results showed that isdb gene sequences were highly conserved, and the recombinant Isdb was successfully expressed. The antibody titer in the immunized groups was increased significantly (P < 0.05) compared with the control, the protective rate of Isdb protein inducted by challenge with the two S. aureus stains Wood46 and HLJ23-1 was 62.5% and 75%, respectively. These results showed that the Isdb protein had high immunogenicity and immunoprotective capacity.
