Kinetics of enzymatic hydrolysis of steam-explosion pretreated corn straw.
- Author:
Shengtao REN
1
;
Keke CHENG
;
Andong SONG
;
Jian'an ZHANG
Author Information
1. College of Life Science, Henan Agricultural University, Zhengzhou 450002, China.
- Publication Type:Journal Article
- MeSH:
Catalysis;
Cellulase;
chemistry;
Hydrolysis;
Kinetics;
Plant Stems;
Steam;
Zea mays
- From:
Chinese Journal of Biotechnology
2011;27(4):592-597
- CountryChina
- Language:Chinese
-
Abstract:
In order to learn the enzymatic hydrolysis characteristics of steam-explosion pretreated corn straw by cellulase, the effects of substrate concentration, cellulase concentration and temperature were determined. The kinetics of the hydrolysis reaction could be described with the Michealis-Menten equation, and the hydrolysis reaction obeyed the classical first-order reaction rate in the first three hours. In the condition of 45 degrees C and pH 5.0 and the stirring rate 120 r/min, the Michealis constant (Km) and maximum rate (Vm) for 1.2 FPU/mL of cellulase were 11.71 g/L and 1.5 g/(L x h). The kinetic model, including the parameters such as substrate concentration, enzymatic concentration and temperature, was suit for the hydrolysis reaction under the temperature range from 30 degrees C-50 degrees C.
