Expression and characterization of a new class IIa bacteriocin.
- Author:
Yan XIE
1
;
Haiqin CHEN
;
Qiuxiang ZHANG
;
Fengwei TIAN
;
Yongquan CHEN
;
Hao ZHANG
;
Wei CHEN
Author Information
1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Anti-Bacterial Agents;
pharmacology;
Bacteriocins;
biosynthesis;
genetics;
Chromatography, Affinity;
Escherichia coli;
genetics;
metabolism;
Genetic Vectors;
genetics;
Green Fluorescent Proteins;
biosynthesis;
genetics;
Listeria monocytogenes;
drug effects;
Molecular Sequence Data;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
pharmacology
- From:
Chinese Journal of Biotechnology
2011;27(7):976-982
- CountryChina
- Language:Chinese
-
Abstract:
NB-Cl gene is a potential class IIa bacteriocin gene. To obtain its soluble expression, NB-C1 was fused with the green fluorescent protein (GFP) gene and a recombinant expression vector plVEX 2.4d-GFP-NB-C1 was constructed, which was transformed into Escherichia coli BL21(DE3) pLysS. The expressed fusion protein GFP-NB-CI was purified by Ni-NTA affinity chromatography and the bioactivity was examined using Listeria monocytogenes as the indicator bacteria. The results showed that the expressed fusion protein GFP-NB-C1 was soluble and the final concentration of the purified fusion protein was 36.1 mg/L E. coli culture and had the purity above 95%. The antimicrobial assay of GFP-NB-C1 was analyzed and showed its high activity against Listeria monocytogenes.
