Preparation of recombinant cutinase and its application in surface modification of poly (ethylene terephthalate).
- Author:
Yao ZHANG
1
;
Sheng CHEN
;
Dan WU
;
Miao HE
;
Kongliang ZHU
;
Jian CHEN
;
Jing WU
Author Information
1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
- Publication Type:Journal Article
- MeSH:
Actinomycetales;
enzymology;
genetics;
Carboxylic Ester Hydrolases;
biosynthesis;
chemistry;
genetics;
Escherichia coli;
genetics;
metabolism;
Fermentation;
Polyethylene Glycols;
chemistry;
Polyethylene Terephthalates;
Recombinant Proteins;
biosynthesis;
genetics;
Surface Properties
- From:
Chinese Journal of Biotechnology
2011;27(7):1057-1064
- CountryChina
- Language:Chinese
-
Abstract:
Fermentation and induction conditions for recombinant Escherichia coli expressing Thermobifida fusca cutinase were optimized in flasks and 3L fermenter. Surface modification of poly (ethylene terephthalate) fibers with cutinase was also discussed. The results showed that, cutinase yield reached 128 U/mL by adding 2 g/L inducer lactose and 0.5% glycine. In the fed-batch culture in a 3 L fermenter, the maximum biomass cutinase activity was up to 506 U/mL, which is the highest bacterial cutinase activity reported by far. Recombinant cutinase was used to modify polyester fibers and terephthalic acid substance was detected by using UV analysis. The dyeing and wetting properties of cutinase treated fibers were higher than untreated fibers. Combined utilization of cutinase and Triton X-100 can significantly improve the hydrophilicity of polyester. This is the first report of surface modification on polyester fibers by bacterial cutinase.
