Cloning of bovine c-myc gene and its expression in skin fibroblast cells.
- Author:
Jiajia XIAO
1
;
Fengfeng ZHANG
;
Xianrong XIONG
;
Yong ZHANG
Author Information
1. College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cattle;
Cloning, Molecular;
Fetus;
cytology;
Fibroblasts;
cytology;
Genetic Vectors;
genetics;
Proto-Oncogene Proteins c-myc;
biosynthesis;
genetics;
RNA, Messenger;
biosynthesis;
genetics;
Recombinant Proteins;
biosynthesis;
genetics;
Skin;
cytology;
Transfection
- From:
Chinese Journal of Biotechnology
2011;27(6):963-968
- CountryChina
- Language:Chinese
-
Abstract:
In order to construct a eukaryotic expression vector of bovine c-myc gene, the coding sequence (CDS) of c-myc gene was amplified from bovine primordial genital ridges by RT-PCR. The CDS was subcloned into pMD19-T vector, and then inserted into vector pIRES2-AcGFP1-Nuc. After confirmed by restriction enzyme digestion and sequencing, the recombined plasmid was transfected into skin fibroblast cells. RT-PCR and Western Blotting were used to detect the expression of c-myc mRNA and protein, respectively. The results show that the complete CDS of c-myc gene was cloned from fetal bovine primordial genital ridges. The eukaryotic expression vector of bovine c-myc gene was constructed and efficiently expressed in the skin fibroblast cells. The present study will lay a good foundation for further study of c-myc gene function and bovine induced pluripotent stem cells from somatic cells by defined factors.
