Preparation of collagen-based materials for wound dressing.
- Author:
Zhigu WU
1
;
Zhiyong SHENG
;
Tongzhu SUN
;
Miao GENG
;
Junyou LI
;
Yongming YAO
;
Zuxiu HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acids; analysis; Animals; Biological Dressings; Cattle; Collagen; analysis; chemistry; isolation & purification; Freeze Drying; Polyurethanes
- From: Chinese Medical Journal 2003;116(3):419-423
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo describe the methods which were used to develop collagen-based materials for wound dressing.
METHODSFresh frozen bovine tendon was treated with 0.05 mol/L acetic acid at pH 3.2 for 48-72 hours, homogenized, filtered, mixed with 8% chondroitin sulphate, for creating a deaerated 1.5%-2.5% collagen solution. The solution was lyophilized in either a pre-frozen or non-pre-frozen mould. The collagen sponge was then cross-linked with 0.25% glutaraldehyde for 24 hours. Three other types of wound dressings were developed using a similar method: collagen membrane with a polyurethane membrane onlay, polyurethane-coated collagen membrane and collagen membrane on gauze.
RESULTSIt was demonstrated that the use of frozen bovine tendon was stable, and that the prepared collagen sponge contained pores of 50-400 microm in diameter.
CONCLUSIONSCollagen could be used as wound dressing.
