Differentiation of rat embryonic neural stem cells promoted by co-cultured Schwann cells.
- Author:
Hong WAN
1
;
Yihua AN
;
Zeshun ZHANG
;
Yazhuo ZHANG
;
Zhongcheng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; Coculture Techniques; Embryo, Mammalian; cytology; Fluorescent Antibody Technique; Microscopy, Phase-Contrast; Nerve Growth Factors; physiology; Neurons; cytology; Phenotype; Rats; Rats, Sprague-Dawley; Schwann Cells; physiology; Stem Cells; cytology
- From: Chinese Medical Journal 2003;116(3):428-431
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the factors which induce differentiation of embryonic neural stem cells.
METHODSRat embryonic neural stem cells were co-cultured with newborn rat Schwann cells in serum-free medium. The phenotype and specific-markers including tubulin-beta, glial fibrillary acidic protein (GFAP) and galactorcerebroside (GalC), were demonstrated by phase contrast microscopy and double immunofluorescence staining.
RESULTSOverall, 80% +/- 5% of neural stem cells protruded several elongated processes and expressed tubulin-beta antigen at high levels, while 20 +/- 3% of them protruded several short processes and were GalC or GFAP positive.
CONCLUSIONThe factors secreted by Schwann cells could induce rat embryonic neural stem cell to differentiate.
