The potential role of IGF-I receptor mRNA in rats with diabetic retinopathy.
- Author:
Hongyu KUANG
1
;
Wei ZOU
;
Dan LIU
;
Rongxing SHI
;
Lihua CHENG
;
Huiqing YIN
;
Xiaomin LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Glucose; analysis; Diabetic Retinopathy; etiology; Glycated Hemoglobin A; analysis; Insulin-Like Growth Factor I; physiology; Male; RNA, Messenger; analysis; Rats; Rats, Wistar; Receptor, IGF Type 1; genetics; Retina; metabolism; pathology
- From: Chinese Medical Journal 2003;116(3):478-480
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo evaluate the potential role of insulin-like growth factor-1 receptor mRNA (IGF-IR mRNA) in the onset and development of retinopathy in diabetic rats.
METHODSA diabetic model was duplicated in Wistar rats. The early changes in the retina were examined using light and transmission electron microscopy. Expression of IGF-IR mRNA was analyzed using in situ hybridization.
RESULTSWeak expression of IGF-IR mRNA (5%) was found in retinas of normal rats, but was significantly increased (15% and 18%) in the retinas of diabetic rats after 3 and 6 months of diabetes (P < 0.01). In situ hybridization and morphological study demonstrated that there was a positive correlation between IGF-IR mRNA expression and retinal changes at various stages.
CONCLUSIONIncreased IGF-IR mRNA might play an important role in the onset and development of diabetic retinopathy.
