Impact of rheumatoid arthritis-associated HLA-DRbeta1 subtypes on protein kinase A signaling.
- Author:
Zhanguo LI
1
;
Lei HAN
;
Rulin JIA
;
Jing LI
Author Information
- Publication Type:Journal Article
- MeSH: Adenylyl Cyclases; analysis; Arthritis, Rheumatoid; etiology; genetics; immunology; Cyclic AMP; analysis; genetics; Cyclic AMP-Dependent Protein Kinases; physiology; Down-Regulation; HLA-DR Antigens; analysis; genetics; Immunophenotyping; In Vitro Techniques; Signal Transduction
- From: Chinese Medical Journal 2003;116(5):712-716
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the impact of rheumatoid arthritis (RA)-associated HLA-DRbeta1 * 0401, * 0402, * 0403, * 0404 and * 0101 subtypes on the protein kinase A (PKA) signaling pathway.
METHODSAdenylate cyclase (AC), cAMP and PKA activity in transfectants expressing RA-associated HLA-DRbeta1 subtypes and their mutants were detected.
RESULTSCompared to HLA-DRbeta1 * 0402 transfectants, the RA-associated HLA-DRbeta1 * 0401, * 0404 and * 0101 transfectants produced significantly lower levels of AC, cAMP and PKA.
CONCLUSIONRA-associated HLA-DRbeta1 molecules are involved in the pathogenesis of RA through down-regulation of the PKA signaling pathway.
