Glucocorticoid modulation of extracellular signal-regulated protein kinase 1/2 and p38 in human ovarian cancer HO-8910 cells.
- Author:
Bing XIA
1
;
Jian LU
;
Gang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Division; physiology; Dexamethasone; pharmacology; Enzyme Activation; Female; Glucocorticoids; pharmacology; Humans; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases; metabolism; Ovarian Neoplasms; enzymology; pathology; Signal Transduction; Tumor Cells, Cultured; p38 Mitogen-Activated Protein Kinases
- From: Chinese Medical Journal 2003;116(5):753-756
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the signaling pathway through testing the effects of dexamethasone (Dex) on the activation of the extracellular signal-regulated protein kinase 1/2 (ERK1/2) and p38 kinase (p38) in HO-8910 cells.
METHODSActivation of the ERK1/2 and p38 was detected by Western blotting using the antibodies against the total ERK1/2 and p38 mitogen-activated protein kinases (MAPKs) protein and the phosphorylated forms of them.
RESULTSDex could suppress the activation of ERK1/2, while enhance the activation of p38 rapidly and strongly in a dose- and time- dependent manner. Neither effect could be blocked by RU486, the antagonist of glucocorticoid receptor (GR).
CONCLUSIONDex has rapid effects on the activation of ERK1/2 and p38, and these effects are not mediated by GR.
