Expression and identification of type 1 diabetes associated autoantigen IA-2.
- Author:
Xiujuan JIA
1
;
Guo LI
;
Zhan CHEN
;
Guangwu XU
;
Chao XIE
;
Di ZHANG
;
Wenzhong ZHOU
;
Sheng ZHENG
;
Xiaoyan XIE
;
Jian YANG
;
Jiping LI
;
Min LUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Autoantigens; biosynthesis; DNA, Complementary; analysis; Diabetes Mellitus, Type 1; immunology; Escherichia coli; genetics; Humans; Membrane Proteins; biosynthesis; genetics; isolation & purification; Plasmids; Protein Tyrosine Phosphatase, Non-Receptor Type 1; Protein Tyrosine Phosphatases; biosynthesis; genetics; isolation & purification; Rabbits; Receptor-Like Protein Tyrosine Phosphatases, Class 8; Recombinant Fusion Proteins; biosynthesis; immunology; isolation & purification
- From: Chinese Medical Journal 2003;116(4):524-528
- CountryChina
- Language:English
-
Abstract:
OBJECTIVESTo obtain prokaryotic expressed IA-2 recombinant protein and to identify its immunological activity.
METHODSThe complimentary DNA (cDNA) coding for the intracytoplasmic part of IA-2 (IA-2ic) was amplified from human fetal brain RNA, and was subcloned into the PinPoint Xa-1 T vector to construct recombinant expression plasmid, and was then expressed in E. coli JM109 cells as a fusion protein with a biotinylated peptide sequence at the aminoterminus. The biotinylated fusion protein was then purified by affinity chromatography and was subsequently dialyzed. Finally, its immunogenicity was evaluated by enzyme linked immunosorbent assay (ELISA).
RESULTSThe purified IA-2ic fusion protein resolved on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as a single Coomassie brilliant blue stained band with a molecular weight of 59 kDa and its immunogenicity was confirmed by ELISA.
CONCLUSIONSE. coli expressed IA-2ic fusion protein has immunological activity. It can be used for detection of IA-2 autoantibodies (IA-2A) and for further studies on type 1 diabetes in future.
