Determination of beta-eudesmol in rhizome of Atractylodes lancea by RP-HPLC.

Author: Yan-Ming CHEN ¹ ; Gui-Xin CHOU ; Zheng-Tao WANG
Author Information

1. Key Laboratory of Standardization of Chinese Medicine, Ministry of Education, Institutes of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Publication Type:Journal Article
MeSH: Atractylodes; chemistry; Chromatography, High Pressure Liquid; methods; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Rhizome; chemistry; Sesquiterpenes, Eudesmane; analysis; standards
From: China Journal of Chinese Materia Medica 2007;32(21):2265-2267
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo develop a RP-HPLC method for determination of beta-eudesmol in rhizome of Atractylodes lancea, and to provide valuble data for quality control of A. lancea.
METHODThe samples were separated on an Inertsil ODS-3 (4.6 mm x 250 mm, 5 microm) column with the mobile phase of acetonitrile-water (68:32). Flow rate was 1.0 mL x min(-1). The detection wavelength was set at 200 nm. Column temperature was 25 degrees C.
RESULTThe contents of beta-eudesmol determinated was 0.833-4.466 mg x g(-1), The linear range of beta-eudesmol was 0.048-1.200 microg (r = 0.999 9), the average recovery was 99.3%, RSD was 1.4% (n = 9).
CONCLUSIONThe method for quantitation of beta-eudesmol in A. lancea was accurate and reliable, which can be used to evaluate the quality of rhizome of A. lancea.