Determination of HPLC fingerprint of Qingfengtong capsule.
- Author:
Yong-Xin ZHANG
1
;
Hua YANG
;
Qi-Wei ZHANG
;
Hong YI
;
Gui-Pin CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Capsules; Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; analysis; isolation & purification; standards; Epimedium; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Salvia miltiorrhiza; chemistry; Sinomenium; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(23):2492-2493
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a fingerprint analysis method of Qingfengtong capsule by HPLC.
METHODThe samples were extracted with 70% ethanol in an ultrasonic bath. The extracts were analyzed at 35 degrees C on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) with 0.1 mol x L(-1) potassium dihydrogen phosphate water-solution as mobile phase A, and methanol as mobile phase B. The analysis followed a linear gradient elution program. Initial condition of the mobile phase was 10% B for 2 minutes; then changed to 90% B in 40 minutes. The flow rate was kept at 1.0 ml x min(-1) and the detector wavelength was 262 nm.
RESULTThe main peaks in fingerprint chromatogram of Qingfengtong capsules were separated fairly well. The results of method validation meet the requirements for the fingerprints.
CONCLUSIONThe established method can be used for the quality control of Qingfengtong capsules.
