Determination of iridoids and triterpenes in herb of Swertia pseudochinesis by RP-HPLC.
- Author:
Wen-Zhe HUANG
1
;
Chun-Hui XU
;
Da-Cheng ZHOU
;
Li-Hua TONG
;
Zu-Guang YE
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Flowers; chemistry; Glucosides; analysis; Iridoid Glucosides; Iridoids; analysis; Oleanolic Acid; analysis; Plant Leaves; chemistry; Plant Roots; chemistry; Plant Stems; chemistry; Plants, Medicinal; chemistry; Pyrones; analysis; Reproducibility of Results; Swertia; chemistry; Triterpenes; analysis
- From: China Journal of Chinese Materia Medica 2007;32(23):2494-2496
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a RP-HPLC method for the determination of swertiamarin, sweroside, gentiopicrin and oleanolic acid in different parts of Swertia pseudochinesis.
METHODA Zorbax SB-C18 (4.6 mm x 250 mm, 5 microm) column was used with acetonitrile-water (10:90) and methnol-water(86:14) at detection wavelengths of 238 nm, 246 nm, 274 nm and 207 nm for swertiamarin, sweroside, gentiopicrin and oleanolic acid respectively. The flow rate was 1.0 mL x min(-1) and the column temperature was 25 degrees C.
RESULTAll of the compounds were based--isolated. The linear ranges of swertiamarin, sweroside, gentiopicrin and oleanolic acid were 0.068 9-0.344 4(r = 0.999 2) , 0.001 1-0.014 0 (r2 = 0. 999 8), 0.001 1-0.013 4 (r2 = 0.999 9) and 0.001 1-0.008 8 mg x mL(-1) (r2 = 0. 999 6), respectively.
CONCLUSIONThe method is simple and accurate, which can be used for quality control of S. pseudochinesis.
