Effect of C21 steroidal glycoside from root of Cynanchum auriculatum on D-galactose induced aging model mice.
- Author:
Shi-Xia ZHANG
1
;
Xin LI
;
Jia-Le YIN
;
Li-Li CHEN
;
Hong-Quan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Aging; drug effects; metabolism; Animals; Brain; drug effects; metabolism; Cynanchum; chemistry; Drugs, Chinese Herbal; isolation & purification; pharmacology; Female; Galactose; toxicity; Glycosides; isolation & purification; pharmacology; Liver; drug effects; metabolism; Male; Malondialdehyde; blood; metabolism; Mice; Mice, Inbred ICR; Myocardium; metabolism; Plant Roots; chemistry; Plants, Medicinal; chemistry; Random Allocation; Steroids; isolation & purification; pharmacology; Superoxide Dismutase; blood; metabolism; Telomerase; metabolism
- From: China Journal of Chinese Materia Medica 2007;32(23):2511-2514
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of C21 steroidal glycoside (CSG) from the root of Cynanchum auriculatum from Jiangsu on D-galactose (D-gal) induced aging model mice.
METHODD-gal aging mouse model was established by cervicodorsal region subcutaneous injection with D-gal once a day for eight successive weeks. The mice in the normal control group (NCG, non-modeled) and the model control group (MCG, modeled but untreated) were treated with 1% CMC-Na. The model mice in the low, middle and high-dose CSG and Vitamin E treated groups were treated with a dose of (10, 20, 40, 100 mg x kg(-1) per day, respectively. The SOD activity, MDA content and telomerase activity in serum, heart, liver and brain tissues of mice were measured.
RESULTCSG could obviously increase the SOD activity and decrease the MDA level in serum, heart, liver and brain tissues in D-gal aging mice (P < 0.01). There was no significant difference between three CSG treated groups and Vitamin E treated groups. In comparison of telomerase activity between MCG and the treated groups, it was shown that there was a significant increase in serum in middle and high dose group, and in heart tissues in CSG and Vit E treated groups, but was not in liver and brain tissue.
CONCLUSIONThis study demonstrates that CSG can antagonize free radical injury, increase the SOD activity and decrease the MDA content of serum, heart, liver and brain in D-gal aging mice, and increase the telomerase activity in serum and heart tissues but not in liver and brain tissue.
