Effects of puerarin on expression of nuclear factor kappaB after cerebral ischemia/reperfusion in rats.
- Author:
Mei-Ping DING
1
;
Fei FENG
;
Hai-Tao HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Brain; drug effects; metabolism; pathology; Immunohistochemistry; Infarction, Middle Cerebral Artery; complications; Isoflavones; isolation & purification; pharmacology; Male; Plants, Medicinal; chemistry; Pueraria; chemistry; Random Allocation; Rats; Rats, Sprague-Dawley; Reperfusion Injury; etiology; physiopathology; Transcription Factor RelA; metabolism
- From: China Journal of Chinese Materia Medica 2007;32(23):2515-2518
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the activation of nuclear factor kappaB (NF-KB) and the influence of puerarin on it after cerebral ischemia-reperfusion in rats.
METHODCerebral ischemia-reperfusion injury was induced by 90 min of middle cerebral artery (MCA) occlusion and followed by 2, 6, 12, 24, 72 h reperfusion. Puerarin or saline was intra-peritoneally injected 1h before MCA occlusion and then the drugs were administered once every six hours. The infarct volume and brain edema were determined by TTC stain. Level of NF-kappaB P65 subunit was determined by immunohistochemistry and western blot.
RESULTImmunohistochemistry revealed the translocation of NF-kappaB. A time course of NF-kappaB induction in brain showed that NF-kappaB P65 subunit obviously increased at 6 h, peaked at 24 h and then decreased by 72 h post-reperfusion. Puerarin decreased the level of NF-kappaB at 24, 72 h after reperfusion. There was a decrease trend in brain infarct volume between puerarin and control.
CONCLUSIONNF-kappaB is translocated and its level is increased after ischemia-reperfusion. Puerarin may attenuate the ischemia-reperfusion injury through inhibition of NF-kappaB activation.
