Study on effect of different combinations of traditional Chinese medicine preparation active parts on metabolism of puerarin in plasma of rats.
- Author:
Ying GUO
1
;
Hai-Tong WAN
;
Li ZHANG
;
Yu WANG
;
Jin HAN
;
Qin YU
;
Yuan-Jiang PAN
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; chemistry; isolation & purification; Animals; Area Under Curve; Brain Ischemia; blood; Drug Combinations; Drug Interactions; Drugs, Chinese Herbal; administration & dosage; chemistry; pharmacology; Flavones; chemistry; isolation & purification; Isoflavones; blood; metabolism; pharmacokinetics; Male; Oils, Volatile; chemistry; isolation & purification; Plants, Medicinal; chemistry; Pueraria; chemistry; Random Allocation; Rats; Rats, Sprague-Dawley; Saponins; chemistry; isolation & purification
- From: China Journal of Chinese Materia Medica 2007;32(23):2531-2534
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo build an experimental method to determine the content of puerarin in plasma, observe the effect of different combinations of traditional Chinese medicine preparation active parts on metabolism of puerarin in plasma of rats suffering cerebral ischemia attack after being orally administered the main active parts of Yangyin Tongnao granules.
METHODAbsorption of puerarin in plasma was extracted by acetonitrile. Using hydroxybenzoate as interior standard, methanol-0.5% acetic acid water solution as mobile phase, ODS C(18) chromatography column (4.6 mm x 150 mm, 5 microm) as stationary phase, puerarin was detected at 248 nm.
RESULTThere is a good linear relationship for puerarin in the range 0.025-3.2 microg x mL-1, RSD < 10%, and the average recovery is 97.6%.
CONCLUSIONThe method is sensitive, reliable, and can be well applied in this experiment. It can provide a method for determination of active components in TCM preparation in vitro. Different compatibility influences metabolism of components.
