Effects of AST and ASI on metabolism of free radical in senescent rats treated by HC.
- Author:
Wei-Zu LI
1
;
Wei-Ping LI
;
Yan-Yan YIN
Author Information
- Publication Type:Journal Article
- MeSH: Aging; drug effects; metabolism; Animals; Astragalus membranaceus; chemistry; Catalase; metabolism; Cell Proliferation; drug effects; Cerebral Cortex; metabolism; Female; Free Radicals; metabolism; Glutathione; metabolism; Glutathione Disulfide; metabolism; Hydrocortisone; toxicity; Interleukin-2; metabolism; Liver; drug effects; metabolism; Male; Malondialdehyde; metabolism; Plants, Medicinal; chemistry; Random Allocation; Rats; Rats, Sprague-Dawley; Saponins; isolation & purification; pharmacology; Spleen; cytology; Superoxide Dismutase; metabolism; Triterpenes; isolation & purification; pharmacology
- From: China Journal of Chinese Materia Medica 2007;32(23):2539-2542
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of Astragaloside (AST) and Astragalus Saponin I (ASI) on metabolism of free radical and immune function in senescent rats treated by HC.
METHODHydrocorisone (HC) was used to estabilsh the aging model in rats. The content of molondialdehyde (MDA), glutathoine (GSH) and oxidized glutathoine (GSSG) in liver and brain was detected according to kit. The activity of Mn superoride dismulase (Mn-SOD) and catalase (CAT) was also surveyed by kit. Concanavalin (ConA) was used to detect the proliferation and interleukin-2 (IL-2) production of splenocytes.
RESULTCompared with HC control, AST and ASI could decrease the content of MDA, GSH and GSSG in liver and brain, increase the activity of Mn-SOD and CAT, and promote the proliferation and interleukin-2 (IL-2) activity of splenocytes.
CONCLUSIONAST and ASI could delay the aging effect in rats treated by HC, and its mechanism maybe the antioxidant and regulating immunity.
