Preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography with macroporous resin.
- Author:
You-Wei XU
1
;
Yan QI
;
Xu HAN
;
Li-Na XU
;
Qi-Wei XU
;
Jin-Yong PENG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Countercurrent Distribution; Gentiana; chemistry; Glucosides; isolation & purification; Iridoid Glucosides; Iridoids; isolation & purification; Plant Roots; chemistry; Plants, Medicinal; chemistry; Resins, Synthetic; Rhizome; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(24):2595-2597
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for the preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography (HSCCC).
METHODThe crude alcohol extracts were eluted on a macroporous resin column and then purified by high speed counter-current chromatography (HSCCC). A two-phase solvent system composed of ethyl acetate: n-butanol: water (2 : 1 : 3) was used, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL x min(-1), while the apparatus rotated at 800 r x min(-1) and the eluate was detected at 254 nm.
RESULT136 mg gentiopicrin with purity of 99.6% determined by HPLC were obtained from 300 mg crude extraction only in one-step separation and less than 200 minutes.
CONCLUSIONThe established method is simple, high efficiency and suitable for large-scale separation of gentiopicrin.
