Fast and simultaneous determination of four index components in Qingwei Huanglian tablets by RP-HPLC.
- Author:
Yan-Rong LI
1
;
Ye JIANG
;
Fu HAO
;
Xiang-Yu DING
Author Information
- Publication Type:Journal Article
- MeSH: Berberine; analysis; Chromatography, High Pressure Liquid; methods; Coptis; chemistry; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Flavonoids; analysis; Gardenia; chemistry; Glycyrrhiza; chemistry; Glycyrrhizic Acid; analysis; Iridoids; analysis; Plants, Medicinal; chemistry; Quality Control; Scutellaria baicalensis; chemistry; Tablets
- From: China Journal of Chinese Materia Medica 2007;32(24):2597-2600
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a RP-HPLC method for fast and simultaneous determination of four index contents, which are Genpioside, Baicalin, Berberine Hydrochloride and Ammonium Glyeyrrhizinate, in Qingwei Huanglian tablets.
METHODA separation was well achieved by gradient elution on a Hypersil C18 (4.6 mm x 250 mm, 5 microm) column with the mobile phases of acetontrile -0.5% triethylamine water-solution (pH 3.0) at a flow rate of 1.0 mL x min(-1), detection wavelength of 230 nm, and room temperature.
RESULTThe calibration curve were linear in the ranges of 4.82-77.2, 5.80-92.8, 1.63-26.1 and 6.40-102 microg x mL(-1) (r = 0.9997-0.9999) for genpioside, baicalin, berberine hydrochloride and ammonium glyeyrrhizinate, respectively. The average recoveries of four index components were not less than 98.0%.
CONCLUSIONThe method is convenient, rapid and accurate. It is suitable for the quality control of Qingwei Huanglian tablets.
