Simultaneous determination of contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides by HPLC.
- Author:
Gulnar SABIR
1
;
Haji Akber AISA
;
Ming-Hui SHI
;
Xiao-Guang JIA
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; analysis; Chromatography, High Pressure Liquid; methods; Plants, Medicinal; chemistry; Quality Control; Quinolizines; analysis; Reproducibility of Results; Seeds; chemistry; Sophora; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(24):2619-2622
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a HPLC method for simultaneous determination of the contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides.
METHODChromatographic condition included a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) column and thmobile phase consisting of a mixture of methanol-0.2% phosphatic acid (7:93). The detection wavelength was at 205 nm. The flow rate was 1.0 mL x min(-1) and column temperature was set at 30 degrees C.
RESULTAll calibration curves showed good linearity (r > or = 0.9997) within test ranges. The mean recoveries were 100.0%, 97.0% and 97.0% respectively.
CONCLUSIONThis method is simple, rapid and accurate, and suitable for quality control of S. alopecuroides seed.
