Protection of polysaccharide ATPS-2 from Armillariella tabescens on immunological liver injury in mice induced by BCG plus LPS.
- Author:
Feng LI
1
;
Ye-Shou SHEN
;
Jin-Bao MA
;
Gen-Hai ZHAO
;
Xin-Qiang SHI
;
Qiao-Yun ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Agaricales; chemistry; Alanine Transaminase; blood; Animals; Aspartate Aminotransferases; blood; B-Lymphocytes; cytology; Cell Proliferation; drug effects; Chemical and Drug Induced Liver Injury; Interleukin-1; blood; Lipopolysaccharides; Liver; metabolism; pathology; Liver Diseases; immunology; metabolism; pathology; Male; Malondialdehyde; metabolism; Mice; Mycobacterium bovis; Nitric Oxide; blood; Polysaccharides; isolation & purification; pharmacology; Protective Agents; isolation & purification; pharmacology; Random Allocation; Superoxide Dismutase; metabolism; T-Lymphocytes; cytology; Tumor Necrosis Factor-alpha; blood
- From: China Journal of Chinese Materia Medica 2007;32(24):2645-2648
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of polysaccharide ATPS-2 from Armillariella tabescens on the immunological liver injury in mice induced by BCG plus LPS.
METHODBCG and LPS were adopted to establish BCG plus LPS liver injury model in mice. The content of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and NO, the activity of superoxide dismutase (SOD) and malondiadehyde (MDA) content of liver homogenate in mice were measured by colorimetric method. The content of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1), on serum were measured by enzyme linked immunosorbent assay (ELISA) , and T- and B-lymphocyte proliferation were measured by MTT. Index of liver, spleen and thymus were calculated after treatment.
RESULTPolysaccharide ATPS-2 from A. tabescens (25, 50, 100 mg x kg(-1)) could obviously reduce the high level of ALT, AST, NO and TNF-alpha, IL-1 on serum, inhibit the high level of MDA, increase the low activity of SOD in liver homogenate and enhance T-and B-lymphocyte proliferation, elevate the spleen, thymic index and decrease liver index of the mice to different extent.
CONCLUSIONPolysaccharide ATPS-2 from A. tabescens had apparently protective effects in the immunological liver injury mice induced by BCG plus LPS.
