Studies on genetic diversity of medicinal Dendrobium by SRAP.
- Author:
Hong-Hong FAN
1
;
Ting-Chun LI
;
Jing QIU
;
Zheng-Peng LI
;
Yi LIN
;
Yong-Ping CAI
Author Information
- Publication Type:Journal Article
- MeSH: Dendrobium; classification; genetics; Genetic Variation; genetics; Nucleic Acid Amplification Techniques; methods; Phylogeny; Plants, Medicinal; classification; genetics; Polymerase Chain Reaction
- From: China Journal of Chinese Materia Medica 2008;33(1):6-10
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the genetic diversity of medicinal Dendrobium by SRAP.
METHODThe genetic diversity of 9 spices Dendrobium was studied by using the optimized SRAP reaction system. The NTSYS software was used to analyze the markers.
RESULTForty primer pairs were selected from 88 amplified 1 782 polymorphic bands with an average of 44.55 polymorphic bands per primer pair. Cluster analysis using UPGMA method based on the data of SRAP amplified bands by 40 primer pairs showed that 9 spices of could be distinguished into two main groups. Jaccard's similarity coefficient ranged from 0.330 2-0.789 2.
CONCLUSIONThe results of this research indicate that SRAP molecular marker is efficient to study the medical Dendrobium genetic diversity.
