Research on phytoestrogenic effects and its mechanism of psoralen.
- Author:
Pi-Wen ZHAO
1
;
Jian-Zhao NIU
;
Ji-Feng WANG
;
Ling-Qiao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Proliferation; drug effects; Estradiol; analogs & derivatives; pharmacology; Female; Ficusin; pharmacology; Flow Cytometry; Humans; Receptors, Estrogen; antagonists & inhibitors; genetics; Receptors, Progesterone; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: China Journal of Chinese Materia Medica 2008;33(1):59-63
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe and evaluate the phytoestrogenic effects and its mechanism of psoralen in estrogen receptor (ER) alpha and beta positive T47D and ishikawa cells.
METHODThe proliferation rate of T47D influenced by 1 x 10(-5) mol x L(-1) to 1 x 10(-9) mol x L(-1) psoralen and that of Ishikawa influenced by 1 x 10(-6) mol x L(-1) and 1 x 10(-7) mol x L(-1) psoralen were analyzed by MTT assay. PR mRNA expression in T47D was quantified by RT-PCR assay. Estrogen receptor antagonist ICI 182, 780 was employed as a tool. ER-alpha and ER-beta expression of T47D was measured by flow cytometry.
RESULTThe proliferation rates of T47D cells treated with 1 x 10(-5) mol x L(-1) to 1 x 10(-7) mol x L(-1) psoralen and ishikawa cells treated with 1 x 10(-6) mol x L(-1) to 1 x 10(-7) mol x L(-1) psoralen were increased significantly. The RT-PCR result showed that 1 x 10(-7) mol x L(-1) and 1 x 10(-6) mol x L(-1) psoralen could increase PR expression in T47D cells. The above effects could be blocked by ICI 182,780. Psoralen could also induce the augment of ER-alpha and ER-beta expression in T47D cells significantly.
CONCLUSIONPsoralen has phytoestrogenic effects. The effects are attained through ER pathway.
