Determination of five primary chemical constituents in Cassia angustifolia by HPLC.
- Author:
Qiu-Ping WU
1
;
Zhu-Ju WANG
;
Li-Ying TANG
;
Mei-Hong FU
;
Yan HE
;
Jing FANG
;
Qian-Feng GONG
Author Information
- Publication Type:Journal Article
- MeSH: Anthraquinones; chemistry; Apigenin; chemistry; Chromatography, High Pressure Liquid; methods; Glucosides; chemistry; Naphthalenes; chemistry; Reproducibility of Results; Senna Extract; Senna Plant; chemistry; Temperature
- From: China Journal of Chinese Materia Medica 2008;33(4):363-365
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determining the content of primary chemical constituents in the leaves of Cassia angustifolia.
METHODThe HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used , acetonitrile-1% acetic acid (10:90-15: 85-18: 82-20: 80-25: 75) in a gradient manner was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees C and detection wavelength at 270 nm.
RESULTThe results showed that 5 effective components all separated well and showed good linearity.
CONCLUSIONThe method was proved to be rapid, sensitive, accurate, credible and repeatable. It can be applied to quality control of Folium Sennae.
