Determination of rubrofusarin gentiobioside in Cassia obtusifolia by HPLC.
- Author:
Li-Ying TANG
1
;
Zhu-Ju WANG
;
Qiu-Ping WU
;
Yan HE
;
Lu-Qi HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Cassia; chemistry; Chromatography, High Pressure Liquid; methods; Chromones; analysis; chemistry; Drugs, Chinese Herbal; chemistry; Glucosides; analysis; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2008;33(4):366-368
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determining the content of rubrofusarin gentiobioside in Cassia obtusifolia.
METHODThe HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, acetonitrile-THF-1% acetic acid (18: 3:79) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees 2 and detection wavelength at 278 nm.
RESULTA good linearity was obtained from 0.1-0.5 microg with r = 0.999 9 for rubrofusarin gentiobioside. The average recovery was 101.1%, and RSD was 2.23% (n = 5).
CONCLUSIONThe method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.
