Tissue culture and plant regeneration of Ixeridium sonchifolium.
- Author:
Jing TAO
1
;
Shi-Hai YANG
;
Meng-Ping ZHANG
;
Yi-Xin TIAN
Author Information
- Publication Type:Journal Article
- MeSH: Asteraceae; growth & development; physiology; Plants, Medicinal; growth & development; physiology; Regeneration; physiology; Tissue Culture Techniques; methods
- From: China Journal of Chinese Materia Medica 2008;33(4):368-371
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the callus induction and plant regeneration of Ixeridium sonchifolium.
METHODBy using the orthogonal experiment design, the medium for callus induction and plant regeneration was optimized.
RESULTThe optimal medium for callus induction was MS + 2, 4-D 1.5 mg x L(-1) +6-BA 1.5 mg x L(-1) +NAA 1.0 mg x L(-1) + IBA 1.5 mg x L(-1) + KT1.5 mg x L(-1), the optimal medium for inducing adventitious bud was MS +2, 4-D 0.2 mg x L(-1) +6-BA 0.5 mg x L(-1) + NAA 0.5 mg x L(-1) + IBA 0.5 mg x L(-1) + KT 0.5 mg x L(-1). Plantlets were rooted on 1/4MS medium supplemented with different concentrations of IBA, and high rooting and survival was achieved when the IBA concentration was 0.1 mg L(-1).
CONCLUSIONAn efficient system for plant regeneration of I. sonchifolium was established.
