Study on enzyme-assisted extraction of polysaccharides from Dioscorea opposita.
- Author:
Yuan ZHANG
1
;
Qiang LIN
;
Jing-Na WEI
;
Hong-Ji ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Dioscorea; chemistry; Hydrogen-Ion Concentration; Polysaccharides; chemistry; isolation & purification; metabolism; alpha-Amylases; metabolism
- From: China Journal of Chinese Materia Medica 2008;33(4):374-377
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo extract polysaccharides from Dioscorea opposita by alpha-amylase and ultrasound.
METHODThe optimum condition of enzyme-assisted extraction has been obtained through orthogonal test according to the yield of polysaccharides.
RESULTA higher yield of polysaccharides was achieved at 55 degrees C, pH 5.5 , with a load of alpha-amylase 10 mg for 1.0 hour, the extraction rate was increased by in compare of sonolysis treatment alone.
CONCLUSIONTo carry out an enzyme treatment before ultrasound--assisted extraction elevated the yield of polysaccharides.
