DNA methylation levels of genome in cFb transdifferentiation induced by SiO2 in rats.
- Author:
Yong-xing WANG
1
;
Wu YAO
;
Bin GAO
;
Yong-cheng YAO
;
Yi-ming WU
;
Chang-fu HAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Transdifferentiation; drug effects; Cells, Cultured; Coculture Techniques; DNA Methylation; Fibroblasts; cytology; drug effects; Genome; drug effects; Lung; cytology; drug effects; Male; Rats; Rats, Sprague-Dawley; Silicon Dioxide; adverse effects
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2012;30(5):326-330
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the DNA methylation levels of genome in cFb transdifferentiation induced by SiO2 in rats.
METHODSThe primary macrophages and fibrocytes of SD rats were co-cultured directly and indirectly, which were exposed to SiO2 at the doses of 25, 50 and 100 g/ml. The transdifferentiation of cFb was identified with immunohistochemical assay. The genomic DNA methylation levels of cFb were detected with HPLC.
RESULTSUnder the condition of indirect co-culture, as compared with control group, the genomic DNA methylation levels of cFb exposed to SiO2 at the doses of 25, 50 and 100 g/ml reduced by 19.9%, 26.9% and 30.3%, respectively (P < 0.05); as compared with cFb exposed to 100 g/ml SiO2, the genomic DNA methylation levels of cFb exposed to 5-aza-dC decreased by 22.0% (P < 0.05). Under the condition of ThinCert(TM) direct co-culture, as compared with control group, the genomic DNA methylation levels of cFb exposed to SiO2 at the doses of 25, 50 and 100 g/ml reduced by 22.2%, 30.2% and 36.7%, respectively (P < 0.05); as compared with cFb exposed to 100 g/ml SiO2, the genomic DNA methylation levels of cFb exposed to 5-aza-dC decreased by 20.6% (P < 0.05).
CONCLUSIONUnder the co-culture condition in vitro, SiO2 could reduce the genomic DNA methylation levels of cFb. The ThinCert(TM) direct co-culture can be used to study the silicosis fibrosis.
