Effect comparison of Wright-Giemsa stain under different conditions and its apply in cell micronucleus test.
- Author:
Chun-hua LIAO
1
;
Huan-feng BIAN
;
Fang XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Azure Stains; Coloring Agents; Hep G2 Cells; Humans; Micronucleus Tests; Staining and Labeling; methods
- From:Chinese Journal of Industrial Hygiene and Occupational Diseases 2012;30(2):139-141
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo apply the Wright-Giemsa stain in micronucleus test and to explore the stain outcomes of Wright-Giemsa dye of various proportions and staining times.
METHODSUse Wright-Giemsa dye, Wright dye (staining time 3 min) and Giemsa dye (staining time 5 min) to stain HepG2 and then observe the staining effect. The Wright-Giemsa dye was applied under 5 different proportions (3:1-1:3) and different staining times (1, 3, 5, 10, 15 min).
RESULTSAfter stained for 3-5 min with the proportion ratio of 3:1 of Wright-Giemsa dye, the HepG2 cells showed much better staining outcomes compared with the single stain of either Wright or Giemsa.
CONCLUSIONSWright-Giemsa stain can be used in cell micronucleus test to obtain good staining outcomes.
