Polymorphism of XRCC1 and chromosome damage in workers occupationally exposed to benzene.
- Author:
Jing ZHANG
1
;
Jian-ping LÜ
;
Chen ZHANG
;
Li-fang ZHOU
;
Yun-jie YE
;
Pin SUN
;
Zhou-xiang CHENG
;
Zhao-lin XIA
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Benzene; adverse effects; DNA Damage; drug effects; genetics; DNA-Binding Proteins; genetics; Humans; Micronuclei, Chromosome-Defective; Micronucleus Tests; Occupational Exposure; Polymorphism, Single Nucleotide; X-ray Repair Cross Complementing Protein 1; Young Adult
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2012;30(6):423-427
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship between the polymorphisms of DNA repair gene (XRCC1 194, 280 and 399) and the chromosomal damage induced by benzene.
METHODSThe chromosomal damage of the peripheral lymphocytes in 459 workers occupationally exposed to benzene and 88 non-exposed controls were detected with cytokinesis-block micronucleus (CBMN) assay. PCR-RFLP technique was used to measure polymorphisms in XRCC1 194, 280 and 399.
RESULTSIt was found that the MN frequency (2.12‰ ± 1.88‰) of the exposed group was significantly higher than that (1.19‰ ± 1.68‰) of the control group (P < 0.05), in the exposed group, the MN frequency (3.00‰ ± 2.76‰) of older workers (> 35 years) was significantly higher than that (2.02‰ ± 1.71‰) of younger workers (≤ 35 years) (P < 0.05). The effect of genetic polymorphisms of XRCC1 on CBMN was not found. The haplotypes AAA/BAA, AAB/AAB, ABA/ABA, ABB/ABB could associated with the increased frequencies of total micronucleus (P < 0.05).
CONCLUSIONBenzene exposure could result in chromosome damage. Age of workers and diplotypes of XRCC1 could associated with chromosomal damage induced by benzene.
