Antimetastatic effects of genistein on salivary adenoid cystic carcinoma in vivo.
- Author:
Hao LIU
1
;
Guang-yan YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; administration & dosage; Carcinoma, Adenoid Cystic; drug therapy; secondary; Female; Genistein; administration & dosage; Lung Neoplasms; drug therapy; secondary; Mice; Mice, Nude; Random Allocation; Salivary Gland Neoplasms; drug therapy; pathology
- From: Chinese Journal of Stomatology 2004;39(5):373-375
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the antimetastatic effects of genistein on salivary adenoid cystic carcinoma (ACC-M) in vivo.
METHODSACC-M cells were exposed to genistein for 3 days. The treated cells and control cells were then injected into the tail vein of 20 nude mice, which were randomized into 2 groups. The mice were sacrificed at the sixth week after implantation. The lung metastasis rates and the number of metastases were detected. Apoptosis index (AI), the expression of VEGF and MMP-9 of the lung metastases were also observed.
RESULTSCompared to the control groups, the number of metastases was significantly reduced in genistein treated group (P < 0.05). The AI of ACC-M cells was higher in the treated group than in the control group (P < 0.05). The level of VEGF and MMP-9 expression were decreased in the treated groups (P < 0.05).
CONCLUSIONSGenistein has a moderate inhibitory effect on metastasis of ACC. Inducing apoptosis, inhibiting the expression of VEGF and MMP-9 are the mechanisms of antimetastatic effects of genistein on ACC-M in nude mice.
