Telomerase SiRNA inhibits KB cell growth in human oral squamous cell carcinoma.
- Author:
Zhi-qiang YANG
1
;
Guang-sheng CHEN
;
Jia-kun CHEN
;
Zhong-liang WU
;
Yi-xiong LEI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Carcinoma, Squamous Cell; metabolism; pathology; Cell Proliferation; Humans; KB Cells; Mouth Neoplasms; metabolism; pathology; RNA, Messenger; biosynthesis; RNA, Small Interfering; genetics; Telomerase; genetics; metabolism; Tumor Cells, Cultured
- From: Chinese Journal of Stomatology 2004;39(5):376-378
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo test the telomerase SiRNA on telomerase mRNA and on KB cell growth of oral squamous cell carcinoma.
METHODSWe synthesized 21-nucleotide SiRNA duplexes with symmetric 2-nucleotide 3' overhangs corresponding to the target sequence (2 657 approximately 2 675 nucleotide downstream of the start codon) of telomerase mRNA. Telomerase activity, cell proliferation, cell cycle and apoptosis were measured after transfection.
RESULTSTwenty one-nucleotide small interfering RNA (SiRNA) duplexes specifically suppressed expression of endogenous telomerase mRNA in human oral squamous carcinoma KB cells. This inhibitory effect lasted only for about 48 h after transfection. Telomerase activity reduction corresponded to the mRNA suppression. Cell proliferation decreased by 30% at 48 h after transfection and lasted for 120 h after treatment. This inhibitory effect resulted from the block of G(1) to S transition. Apoptosis was not involved in this process.
CONCLUSIONSSiRNA is a powerful tool for studying gene function and can be used as gene-specific therapeutics.
