Expression of pRb and E2F-1 and telomerase activity in ameloblastoma.
- Author:
Ming ZHONG
1
;
Jie WANG
;
Bo ZHANG
;
Lin HOU
;
Yang-li YUE
;
Zi-juan LI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Ameloblastoma; metabolism; pathology; Child; Cyclin E; biosynthesis; E2F1 Transcription Factor; biosynthesis; Female; Humans; Jaw Neoplasms; metabolism; pathology; Male; Middle Aged; RNA, Messenger; biosynthesis; Retinoblastoma Protein; biosynthesis; Telomerase; genetics; metabolism
- From: Chinese Journal of Stomatology 2004;39(5):406-409
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of pRb and E2F-1, and the association between their expression and the activity of telomerase (hTERT) or cyclin E in human ameloblastoma (AB), and to explore the clinical biological characteristics of AB.
METHODSThe expressions of pRb, E2F-1, cyclin E and hTERT mRNA in human AB were detected by in situ hybridization or immunohistochemistry (SP method).
RESULTSThe positive expression ratio of pRb in the cell nucleus of AB was 20.4% (11/54). The positive ratio of E2F-1, cyclin E and hTERT mRNA was 92.6% (50/54), 66.7% (36/54) and 94.4% (51/54), respectively. With AB recurrence and malignant transformation, the expression of hTERT, E2F-1, cyclin E was up-regulated. hTERT and cyclin E or E2F-1 mRNA had high positive relation (Spearsman'r(s) = 1.000, P = 0.0001).
CONCLUSIONSThe regulatory pathway of Rb/E2F-1 is associated with the cell proliferation and in differentiation of AB. The activity or release of telomerase may be related to the lower expression of Rb and higher expression of E2F-1, and is up-regulated in G(1) late phase by cyclin E.
