Interaction of vascular endothelial growth factor-C over-expression with tongue squamous cell carcinoma cell line Tca8113 with peri-carcinoma lymphatics.
- Author:
Hong-zhang HUANG
1
;
Hai-kuo TANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Squamous Cell; pathology; Cell Line, Tumor; Chick Embryo; Humans; Lymph Nodes; pathology; Lymphatic Metastasis; Lymphatic Vessels; drug effects; pathology; Neck; Neoplasm Transplantation; Tongue Neoplasms; pathology; Transfection; Vascular Endothelial Growth Factor C; genetics
- From: Chinese Journal of Stomatology 2005;40(2):126-128
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the role of Vascular endothelial growth factor C (VEGF-C) in the interaction of tongue squamous cell carcinoma (TSCC) with the peri-carcinoma lymphatics.
METHODSTSCC cells were implanted on the chorioallantoic membrane (CAM), in which, group A was transfected by plasmid pREP7 including VEGF-C, group B was transfected by plasmid pREP7, and group C was Tca8113. Lymphatic vessels were stained by 5'-Nase. Morphologic analysis was used to evaluate the alteration of lymphatic vessels density (LVD), area and perimeter.
RESULTSThe transplanted tumor well on CAM. The expression of VEGF-C in group A was higher than that in control group B and C. The LVD, perimeter and area in group A were (5.3 +/- 0.41)/high-power field, (148 +/- 21) microm and (76.8 +/- 13.5) microm(2) respectively in group A, which were significantly higher than that of group B and C (P < 0.01), while there were no significant difference between group B and C.
CONCLUSIONSCAM is ideal model for the research of lymphatic vessels; Over-expression of VEGF-C could induce the dilatation and LVD increase of peri-tumor lymphatic vessels, which maybe one of the mechanisms that VEGF-C metastasis of TSCC through lymphatic vessels.
