Effects of rhizoma paridis total saponins on levels of cytokines in blood serum of two-hit rat model induced by multiple fractures and lipopolysaccharide.
- Author:
Man-hong ZHOU
1
;
Hua-jing HE
;
Yong PAN
;
Li-jing SHAN
;
Yi MA
;
Yang LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cytokines; blood; Female; Fractures, Bone; blood; drug therapy; metabolism; Lipopolysaccharides; metabolism; Male; Multiple Trauma; blood; drug therapy; metabolism; Rats; Rats, Wistar; Rhizome; chemistry; Saponins; pharmacology; therapeutic use
- From: China Journal of Orthopaedics and Traumatology 2008;21(9):662-663
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of rhizoma paridis total saponins(RPTS)on the levels of tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) in blood serum of two-hit rat model induced by multiple fractures and lipopolysaccharide.
METHODSSixty-eight Wistar rats were randomly divided into five groups. The models were made in four groups (expect the blank control group) in accordance with the standard of two-hit animal model induced by multiple fractures and lipopolysaccharide. At 1 hour after models made,the rats in RPTS groups were given rhizoma paridis total saponins with different concentrations by intragastric administration. Six hours later, the concentrations of TNF-alpha, IL-1 beta and IL-6 in the blood serum of all rats were detected by ELISA (enzyme linked immunosorbent assay).
RESULTSThe concentrations of TNF-alpha, IL-1 beta and IL-6 in blood serum of rats in the model group were remarkably higher than those in the blank control group (P<0.001), and these in the RPTS groups were remarkably lower than those in the model group (P<0001).
CONCLUSIONRPTS can decrease the levels of TNF-alpha and IL-6 and IL-1 beta in the blood serum of rats subjected to two-hit induced by multiple fractures and lipopolysaccharide.
