Ghrelin down-regulates ACAT-1 in THP-1 derived foam cells via growth hormone secretagogue receptor-dependent pathway
10.3760/cma.j.issn.0253-3758.2009.11.021
- VernacularTitle:脑肠肽Ghrelin通过生长激素促分泌素受体下调人源单核细胞株THP-1源性泡沫细胞酰基辅酶A:胆固醇酰基转移酶1表达的实验研究
- Author:
Jing-Jing WAN
1
;
Bei CHENG
;
Yan-Fu WANG
;
Chun-Li MEI
;
Wei LIU
;
Li KE
;
Ping HE
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
Atherosclerosis;
Foam cells;
Ghrelin
- From:
Chinese Journal of Cardiology
2009;37(11):1030-1034
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Ghrelin on the expression of acyl coenzyme A: cholesterol acyhransferases-1 (ACAT-1) in THP-1 derived foam cells. Methods The human monocytic leukemia cell line (THP-1) was chosen in our study. The differentiation of THP-1 cells into macrophages was induced by phorbol 12-myristate 13-acetate. Macrophages were then incubated with oxidized LDL (ox-LDL) to generate foam cells. Ghrelin and [D-Lys3]-GHRP-6, the special antagonist of growth hormone secretagogue receptor (GHS-R), were treated during foam cells formation. The ACAT-1 protein and mRNA levels were detected by Western blot and RT-PCR. The effect of variance of cholesterol content was measured by zymochemistry via-fluorospectrophotometer. Results Ghrelin reduced the content of cholesterol ester in foam cells obviously. ACAT-1 protein and mRNA levels were also decreased. The antagonist of GHS-R inhibited the effects of Ghrelin on ACAT-1 expression in dose-dependent manner. The ACAT-1 mRNA levels of the GHS-R specific antagonist groups (10~(-5), 5×10~(-5), 10~(-4) mol/L) were 1.14±0.04、1.58±0.03、 2.40±0.16, significantly higher than that of the Ghrelin group (0.89±0.05). And the protein expressions were 1.25±0.09,1.77±0.11,2.30±0.09, also higher than that of the Ghrelin group (0.86±0.08). Conclusions Ghrelin might interfere atherosclerosis by down-regnlating the expression of ACAT-1 via GHS-R pathway.
