Association between expression of cbormogranin A and myocardial fibrosis in patients with dilated cardiomyopathy
10.3760/cma.j.issn.0253-3758.2009.12.006
- VernacularTitle:扩张型心肌病中嗜铬粒蛋白A的表达及其与心肌纤维化的关系
- Author:
Ye-Qing XIE
1
;
Rui-Zhen CHEN
;
Yong YU
;
Ping CHEN
;
Zhao-Hua YANG
;
Qi GUO
;
Ying-Zhen YANG
Author Information
1. 复旦大学附属中山医院
- Keywords:
Cardiomyopathy;
dilated;
Chromogranin A;
Fibrosis;
Myocardium
- From:
Chinese Journal of Cardiology
2009;37(12):1081-1084
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the possible correlation between expression of chormogranin A (CGA) and myocardial fibrosis and investigate the potential role of CGA in the development of myocardial fibrosis in patients with dilated cardiomyopathy ( DCM ). Methods Surgical myocardial specimen from 10 DCM patients underwent successful orthotopic cardiac transplantation, and 3 normal myocardial specimen from brain-dead organ donors were obtained. CGA-mRNA,COL I -mRNA, COL Ⅲ-mRNA and ADAMTS-1-mRNA were analyzed by real-time PCR. The location and expression of CGA were assessed by immunohistochemistry( INH) with anti-CGA antibody. The collagen specific picrosirus red staining was applied on transversal myocardial slides and the collagen volume fraction ( CVF) was calculated. The correlation between CGA and CVF was analyzed. Results Cytoplasic expression of CGA assessed by INH showed large amount of strong positive granules densely arranged in the epicardial and endocardial myocardiocytes in DCM specimen while there was only few sparse granules in the normal myocardium (P < 0.05). CVF was significantly higher in DCM myocardial specimen than that in normal specimen (P < 0. 001). CGA-mRNA was significantly correlated with COL I -mRNA (r = 0. 729), COL Ⅲ -mRNA (r = 0.95) and ADAMTS-1-mRNA (r =0.665, all P<0.05). Moreover, collagen deposition location was almost identical with the strong positive expression location of CGA. Conclusion We demonstrated for the first time that the deposition of CGA was related with the myocardial fibrosis in DCM heart, therefore, CGA might play an important role by influencing myocardial remodeling and fibrosis in DCM patients.
