The relationship between uncoupling protein 2 expression and myocardial high energy phosphates content in abdominal aorta constriction induced heart failure rats
10.3760/cma.j.issn.0253-3758.2009.12.013
- VernacularTitle:压力超负荷致心力衰竭大鼠解耦联蛋白2表达及心肌能量变化
- Author:
Nan LI
1
;
Jiang WANG
;
Feng GAO
;
Ai-Min LI
;
Rong SONG
;
Ying TIAN
;
Shan-Jun ZHU
Author Information
1. 第三军医大学附属新桥医院
- Keywords:
Heart failure;
congestive;
Mitochondria;
Energy metabolism;
Uncoupling protein 2
- From:
Chinese Journal of Cardiology
2009;37(12):1108-1112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of expression of uncoupling protein 2 (UCP2) in pressure overload induced failure myocardium in rats. Methods Male SD rats were randomized into 3 groups ( n = 15 each): abdominal aorta constriction ( AC) 20 weeks group ( H20w group) , sham operation group (SH20w group) and normal control group (N group). Twenty weeks later, myocardial function was evaluated by echocardiography and hemodynamic measurements. Mitochondria in ventricular tissue were isolated by centrifugation. Adenine nucleotide pools (ATP, ADP, AMP, PCr) in myocardium were measured by high performance liquid chromatography. The expression of UCP2 in mitochondria was detected by PT-PCR and Western blot analysis. Results Myocardial function was significantly decreased 20 weeks post-AC compared to SH20w group and N group. Myocardial ATP, ADP, AMP and PCr contents were also significantly decreased in H20w group than the other 2 control groups. The expression of UCP2 in myocardial mitochondria was significantly increased in H20w group and negatively correlated with ATP contents (r= -0.929,P<0. 01). Conclusions The expression of UCP2 was upregulated in pressure overload induced failure heart and might be responsible for decreased myocardial adenine nucleotide and energy metabolism disturbance in this model.
