Expression of PAH-DNA adducts in lung tissues of Xuanwei female lung cancer patients.
- Author:
Kaiyun YANG
1
;
Yunchao HUANG
;
Guangqiang ZHAO
;
Yujie LEI
;
Kun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Air Pollution, Indoor; adverse effects; Coal; DNA Adducts; analysis; Female; Humans; Lung; chemistry; Lung Neoplasms; chemistry; etiology; Male; Middle Aged; Polycyclic Aromatic Hydrocarbons; analysis
- From: Chinese Journal of Lung Cancer 2010;13(5):517-521
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND AND OBJECTIVEThe coal-fired pollution in Xuanwei area has been considered to be local main reason for high incidence of female lung cancer. The aim of this study is to explore the expression of PAH-DNA adducts in lung tissues of Xuanwei female lung cancer patients and to explore the relationship between the large number of coal-fired pollution PAHs materials and the high incidence of Xuanwei female lung cancer.
METHODSWe totally collected each 20 cases of Xuanwei female lung cancer patients, Xuanwei male lung cancer patients, Non-Xuanwei female lung cancer patients and collect each 10 cases of Xuanwei, Non-Xuanwei female patients with benign lung lesions. The cancer tissues, adjacent cancer tissues and normal lung tissues were collected in lung cancer patients and only the normal tissues were collected in benign lung lesion patients. There were total 80 cases and 200 tissues. Immunofluorescence was used to detect the expression of PAH-DNA adducts in each group. Image pro-plus 6.0 software was used to analyze the images and part quantified analysis. SPSS 13.0 statistical software was used to analyze the data.
RESULTSThe positive expression of PAH-DNA adducts in lung cancer tissues, adjacent cancer tissues and normal lung tissues of Xuanwei female lung cancer patients were 90%, 80% and 65%. They were higher than the positive expression of PAH-DNA adducts in Xuanwei male lung cancer patients (35%, 30%, 30%) and Non-Xuanwei female lung cancer patients (20%, 15%, 10%) (P < 0.01). The expressions in lung tissues ofXuanwei female benign lung lesion patients (positive expression is 70%) were higher than it in Non-Xuanwei female benign lung lesion patients (positive expression is 10%). With the direction changing from cancer tissues, adjacent cancer tissues to normal lung tissues, the expressions of PAH-DNA adducts were decreased but had no statistical difference (P > 0.05S).
CONCLUSIONThe expressions of PAH-DNA adducts in lung tissues of Xuanwei female were higher than which in Xuanwei male and Non-Xuanwei female.
