Anti-inflammatory and analgesia effects of electroacupuncture device of point injection on rats of inflammatory pain.

Yueyuan Fan; Guofu Huang; Fang Gao; Caihua WU; Xiaocui Yuan; Hongping LI; Xiaoli Pan; Wei Chen; Yang Cao; Ludong Xin; Man LI

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Anti-inflammatory and analgesia effects of electroacupuncture device of point injection on rats of inflammatory pain.

Author: Yueyuan FAN ¹ ; Guofu HUANG ¹ ; Fang GAO ² ; Caihua WU ² ; Xiaocui YUAN ² ; Hongping LI ² ; Xiaoli PAN ³ ; Wei CHEN ⁴ ; Yang CAO ⁵ ; Ludong XIN ⁵ ; Man LI ²
Author Information

1. Traditional Chinese Integrated with Western Medicine Hospital, Hubei University of TCM, Wuhan 430022, China.
2. Department of Neurobiology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province.
3. Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology.
4. Wuhan Kangbenlong Medical Equipment Limited Company.
5. Suzhou Medical Appliance Factory.
Publication Type:Journal Article
Keywords: IL-1β; acupuncture analgesia; electroacupuncture (EA); inflammatory pain; point injection (PI)
From: Chinese Acupuncture & Moxibustion 2016;36(8):845-850
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore the anti-inflammatory and analgesia mechanism of electroacupuncture (EA) device of point injection (PI) on rats of inflammatory pain.
METHODS48 Sprague Dawley (SD) rats were randomly assigned into a control group, a model group, an EA+PI group, an EA device of PI (EAPI) group, an EA group and a PI group, eight rats in each one. The rats in the control group were subcutaneously injected with 50 μL of liquid paraffin oil solvent into the dorsum of left hindpaw, while rats in the remaining groups were treated with 50 μL of complete freund's adjuvant (CFA) at identical location to induce the model of inflammatory pain. After model establishment, the rats in the EA+PI group, EAPI group, EA group and PI group were treated with EA+PI,EA device of PI, EA and PI, respectively, once every other day (the 2nd day, 4th day and 6th day). Each treatment was given for 30 min. The mechanical withdrawal threshold, thermal withdrawal threshold and foot swelling before and 1 d to 6 d after model establishment were observed; the western blotting method was applied to measure IL-1β expression in inflammatory tissue of skin.
RESULTSAfter model establishment, compared with the control group, the mechanical withdrawal threshold and thermal withdrawal threshold were reduced (all<0.05) and the foot swelling was increased in the rest groups (all<0.05). After treatment, the mechanical withdrawal threshold and thermal withdrawal threshold in the EAPI group were significantly increased compared with those in the EA+PI group, EA group and PI group (all<0.05), but the foot swelling was reduced (all<0.05). The IL-1β expression in the model group was higher than that in the control group (<0.05); after treatment, the IL-1β expression in the EAPI group was lower than that in the model group, EA group and PI group (all<0.05), but no significantly different from that in the EA+PI group (>0.05).
CONCLUSIONSThe efficacy of EA device of PI on inflammatory pain is superior to EA combined with PI, EA alone and PI alone, which is suitable for further popularization and application.