Immunophenotypic comparison of adipose-derived mesenchymal stem cells from the greater omentums and subcutaneous adipose tissues of rats.
- Author:
Dong-fei LI
1
;
Chun YANG
;
Zhen LI
;
Ling-ling YUAN
;
Jing-xing DAI
;
Lin YUAN
Author Information
- Publication Type:Journal Article
- MeSH: Adipose Tissue; cytology; immunology; Animals; Cells, Cultured; Immunophenotyping; Mesenchymal Stromal Cells; cytology; immunology; Omentum; cytology; immunology; Rats; Rats, Sprague-Dawley; Subcutaneous Fat; cytology; immunology
- From: Journal of Southern Medical University 2010;30(10):2256-2262
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the adipose-derived mesenchymal stem cells (ADMSCs) isolated from the greater omentums and subcutaneous adipose tissues of rats for their characteristics in cell morphology, growth kinetics and immunophenotypes.
METHODSADMSCs were isolated from the greater omentums and inguinal fat pads of 6 SD rats and cultured in vitro. The morphologies of the ADMSCs were observed using phase-contrast microscopy, and their growth curves were generated and the doubling times determined. The phenotypic marker profiles including CD11b, CD29, CD45, CD49d, CD90 and CD106 of the ADMSCs in the fourth passage were determined using flow cytometry.
RESULTSThe ADMSCs harvested from the greater omentums and inguinal fat pads showed almost identical morphologies. The growth curves and the mean doubling time of the ADMSCs from the two different sources showed no obvious difference. With similar positivity rates for CD11b, CD29, CD106 and CD90, the two ADMSCs exhibited different expression rates of CD45 and CD49d.
CONCLUSIONSThe immunophenotypic characteristics of the ADMSCs from the greater omentums and subcutaneous adipose tissues are not totally identical.
