Variations of the amount of sialic acids on hepatocellular carcinoma cell membrane.
- Author:
Zu-yuan GONG
1
;
Cai-xian LIAO
;
Yu WANG
;
Xin-xin LIAO
;
An-cheng QIN
;
Yong-ping HUANG
;
Hui LIAO
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; metabolism; pathology; Cell Membrane; metabolism; Humans; Liver Cirrhosis; metabolism; pathology; Liver Neoplasms; metabolism; pathology; Sialic Acids; metabolism
- From: Journal of Southern Medical University 2010;30(10):2323-2326
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the change in the amount of sialic acids on hepatocellular carcinoma (HCC) cell membrane.
METHODSSurgical specimens of HCC and liver cirrhosis tissues were obtained from 28 patients to prepare carcinoma cell and hepatocyte suspensions by collagenase digestion. For assay of α2, 3 and α2, 6-sialic acids, the cells were suspended in the staining buffer containing either fluorescein isothiocyanate-Maackia amurensis lectin (FITC-MAL) or fluorescein isothiocyanate-Sambucus nigra bark lectin (FITC-SNA) and incubated for 1 h, respectively. Flow cytometric analysis was carried out to measure the mean fluorescence intensity (MFI) on the cell surface.
RESULTSIn both FITC-MAL- and FITC-SNA-incubated HCC cells, the MFI on the cell surface was greater than that of the hepatocytes.
CONCLUSIONBoth of α2, 3 and α2, 6- sialic acids increases significantly on the hepatocyte membrane after the carcinomatous change.
